LOCUS       40924_49292        3410 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD063, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3410)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    408..428
                     /note="G5C5; GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons; synthetic"
     misc_feature    429..434
                     /label=XbaI
                     /note="XbaI"
     oriT            complement(493..602)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    739..744
                     /label=SacI
                     /note="SacI"
     protein_bind    758..779
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        794..824
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    832..848
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             868..1041
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     misc_feature    1099..1104
                     /label=SacI
                     /note="SacI"
     misc_feature    1105..1125
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons or Escherichia coli origins to be 
                     assembled with a cyanobacterial replicon; synthetic"
     misc_feature    1123..1128
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    1147..1152
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(1189..1205)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1213..1229)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1237..1267)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1282..1303)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1591..2179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2353..3210)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3211..3315)
                     /label=AmpR promoter
     misc_feature    3341..3346
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgaggatatc gggggccccc
      421 ggggggattc tagactggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
      481 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
      541 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
      601 ccggctgacg ccgttggata caccaaggaa agtctacgcg aaccctttgg caaaatcctg
      661 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
      721 gttatgcagc ggaagatcga gctcagcgca acgcaattaa tgtgagttag ctcactcatt
      781 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      841 gataacaatt tcacacagga aacagctatg accatgatta cggattcact ggccgtcgtt
      901 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
      961 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1021 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     1081 gaaagctggc tggagtagga gctcatcccc gggcccgggc ccgatatcct cggtacccgg
     1141 ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt
     1201 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1261 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1321 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1381 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1441 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2401 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     3361 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
